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human platelet non muscle actin  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc human platelet non muscle actin
    Human Platelet Non Muscle Actin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+platelet+non+muscle+actin/Actin+protein+human+platelet+beta+gamma+actin/pm42745469-412-1-20
    Average 95 stars, based on 187 article reviews
    human platelet non muscle actin - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: A Legionella Effector Disrupts Host Cytoskeletal Structure by Cleaving Actin
    Article Snippet: Signals from each individual protein were detected by fluorescence dye-conjugated antibodies on an Odyssey detection system (Li-Cor). .. Rabbit skeletal muscle actin (>99% pure) (Cytoskeleton) or Human platelet non-muscle actin (>99% pure) (Cytoskeleton) were incubated with RavKΔC50 or RavK H95A ΔC50 in G-actin buffer (5 mM Tris-HCl 8.0, 0.2 mM CaCl 2 , 0.2 mM ATP, 0.5 mM DTT) at 22°C for indicated time periods. ..

    Article Title: A Legionella Effector Disrupts Host Cytoskeletal Structure by Cleaving Actin.
    Article Snippet: Signals from each individual protein were detected by fluorescence dye-conjugated antibodies on an Odyssey detection system (Li-Cor). .. In vitro cleavage assay Rabbit skeletal muscle actin (>99% pure) (Cytoskeleton) or Human platelet non-muscle actin (>99% pure) (Cytoskeleton) were incubated with RavKΔC50 or RavKH95AΔC50 in G-actin buffer (5 mM Tris-HCl 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 0.5 mM DTT) at 22 ̊C for indicated time periods. ..

    Concentration Assay:

    Article Title: Sephin1 rewires proteostasis through actin-dependent signaling
    Article Snippet: Digital electron micrographs taken with iTEM-FIVE software (EMSIS) were optionally processed with Adobe Photoshop V.9, namely adjustment of contrast and brightness, gray-scale modification, sharpening, median and high-pass filtering. .. Human platelet non-muscle actin (Cytoskeleton Inc., APHL99-C) was diluted to a final concentration of 250 nM in the actin buffer [5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran]. .. Human platelet non-muscle actin (Cytoskeleton Inc., APHL99-C) was diluted to a final concentration of 250 nM in the actin buffer [5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran].

    Article Title: Sephin1 rewires proteostasis through actin-dependent signaling
    Article Snippet: .. Human platelet non-muscle actin (Cytoskeleton Inc., APHL99-C), rabbit skeletal muscle actin (Cytoskeleton Inc., # AKL95) or BSA (Sigma, A9647) were diluted to the concentration indicated in the text in the following actin buffer [2.5 mM Tris-HCl pH 8.0, 0.1 mM CaCl2, 0.1 mM ATP, 2.5% (w/v) sucrose, 0.5% (w/v) dextran]. .. Human platelet non-muscle actin (Cytoskeleton Inc., APHL99-C), rabbit skeletal muscle actin (Cytoskeleton Inc., # AKL95) or BSA (Sigma, A9647) were diluted to the concentration indicated in the text in the following actin buffer [2.5 mM Tris-HCl pH 8.0, 0.1 mM CaCl2, 0.1 mM ATP, 2.5% (w/v) sucrose, 0.5% (w/v) dextran].

    In Vitro:

    Article Title: A Legionella Effector Disrupts Host Cytoskeletal Structure by Cleaving Actin.
    Article Snippet: Signals from each individual protein were detected by fluorescence dye-conjugated antibodies on an Odyssey detection system (Li-Cor). .. In vitro cleavage assay Rabbit skeletal muscle actin (>99% pure) (Cytoskeleton) or Human platelet non-muscle actin (>99% pure) (Cytoskeleton) were incubated with RavKΔC50 or RavKH95AΔC50 in G-actin buffer (5 mM Tris-HCl 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 0.5 mM DTT) at 22 ̊C for indicated time periods. ..

    Cleavage Assay:

    Article Title: A Legionella Effector Disrupts Host Cytoskeletal Structure by Cleaving Actin.
    Article Snippet: Signals from each individual protein were detected by fluorescence dye-conjugated antibodies on an Odyssey detection system (Li-Cor). .. In vitro cleavage assay Rabbit skeletal muscle actin (>99% pure) (Cytoskeleton) or Human platelet non-muscle actin (>99% pure) (Cytoskeleton) were incubated with RavKΔC50 or RavKH95AΔC50 in G-actin buffer (5 mM Tris-HCl 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 0.5 mM DTT) at 22 ̊C for indicated time periods. ..



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